We harvest stem cells from your pet’s fat stores. Stem cells are plentiful in the abdominal fat of the pet. We make a small, barely visible incision and collect a small amount of fat for processing.
We then separate the stem cells from the fat and concentrate the cells for injection. The fat is mixed with enzymes that separate the stem cells and placed in a special heated centrifuge. The process takes about 90 minutes.
Once separated, the cells are stimulated back into activity by adding platelet rich plasma and, in some cases, using laser to further enhance their activity. The number of live cells is used to calculate the volume of dose.
The stem cells are injected into the area of concern and immediately start the process of healing, by reducing inflammation and regrowing tissue.
Stem cells are “mother” cells giving rise to “daughter” cells, which differ from the parent. These differences are dictated by the needs of the body for repair. “Mother” stem cells placed into an arthritic joint, for example, will be bathed in the joint fluid that contains chemicals released from the diseased cartilage cells. These chemicals help direct the development of the “daughter” cells into new cartilage cells. These new cells replace the missing or damaged cells with new cartilage. This is regeneration, not healing. Healing results in the formation of scar tissue, or fibrous replacement tissue, that is not as functional as the original tissue. Stem cells regenerate the original cartilage tissue, just as it was when the animal was an infant.
Harvest, Process & Prepare
A small incision in the abdomen allows us to collect a small amount of fat filled with stem cells.
The fat is now sent to the laboratory for preparation.
The fat is mixed with enzymes that separate the stem cells and placed in a special heated centrifuge.
The centrifuge first mixes the cells then separates them from the remaining fat.
The process takes about 90 minutes.
A special kit for stem cell separation is used.
The fat can be seen at the top and the stromal vascular fraction can be seen at the bottom containing stem cells.
After several washes and a filtration process the stem cells are in the pellet at the bottom of the tubes.
The cells are counted with special dyes that tell live stem cells from dead ones.
The hemacytometer is a carefully crafted microscope slide that’s made from thick glass with a rectangular indention that creates a chamber. Etched on the glass surface of the chamber is a defined gridded area of squares with specific area and depth. Thereby, it is possible to count the number of cells in a specific volume of fluid.
The hemacytometer is read using a special fluorescence illuminator device called a Bioscope™. Therefore, cells that are loaded on the hemacytometer are illuminated by ultraviolet light for optical analysis.
Using a microscope, we can determine the number of cells by direct counting.
The number of live cells is used to calculate the volume of dose.
This is the final stem cells mixed with platelet rich plasma ready for injection